Research by: University of Texas at Arlington, US. One of the leading groups in photoactive drug discovery. Developed first clinical-level rubidium-based PS (TLD-1433), now in Theralase-sponsored Phase 2 trials for bladder cancer.
Modulight products: ML8500 (445 nm, 525 nm, 630 nm, 753 nm, 810 nm)
Laser use: Photoactive drug discovery
Link to the study:
Prof. Sherri McFarland
Research topics: Medicinal inorganic chemistry and drug discovery. Special interest in the synthesis of novel transition metal complexes and studying their interactions with light for the purpose of exploiting those interactions for PDT and photochemotherapy. Rational manipulation of photophysics of the molecules to produce very potent photosensitizing effects, especially in hypoxic conditions and with varying wavelengths.
Other collaborations with Acadia University, UNC at Greensboro, Wake Forest Cancer Center & Roswell Park.
Sherri McFarland discussing how ML8500 is supporting her research
Study protocol to determine wavelength, fluence, and irradiance activities of Os-4T:
- Lead in compound family (Os-4T) was applied to SK-MEL-28 melanoma cells in well-plates
- Well-by-well illuminations in triplicates with the ML8500 (37 ⁰C, 5% CO2) using 525 nm and 630 nm wavelengths. In the first experiment, irradiance was kept constant at 300 mW/cm2 and fluence was varied between 10-300 J/cm2, while in the second experiment, fluence was constant at 100 J/cm2 and irradiance varied 25-300 mW/cm2.
- Determine cell viability with Resazurin assay and read absorbance at 570 and 600 nm. Resazurin assay principle: The irreversible reaction of the purple resazurin to the pink-colored resorufin is proportional to aerobic respiration, indicating the amount of viable cells.
Key observations
Illumination parameters significantly affected the phototoxicity of Os-4T. The green light (525 nm) was more effective than the red light (630 nm) at lower fluences, while the maximal potency was achieved at both wavelengths when using fluences higher than 100 J/cm2. Irradiance affected the potency with both the red and the green light that were more effective when lower irradiances were used.
Graphs from the original publication. Open-access content reproduced with permission from the contact author of the publication.
Related Modulight products and Services
ML8500 – Automated Illumination for Microplates »
Related Publications
Breaking the barrier: an osmium photosensitizer with unprecedented hypoxic phototoxicity for real world photodynamic therapy
John A. Roque, III, Patrick C. Barrett, Houston D. Cole, Liubov M. Lifshits, Ge Shi, Susan Monro, David von Dohlen, Susy Kim, Nino Russo, Gagan Deep,d Colin G. Cameron, Marta E. Alberto and Sherri A. McFarland
Chemical Science, 2020, 36
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