Biolab

Modulight biomedical laboratories

We're at the forefront of driving customer research and development. Our expertise is your fast track to market success, whether you're developing groundbreaking cancer drugs, harnessing laser technology, or conducting in-depth in vitro studies with cancer cell lines. Backed by a multidisciplinary team of engineers, biotechnology experts, and medical professionals, we're here to guide your journey.

Modulight's BIOLAB at your service

General biomedical laboratory

  • Fluorescence feasibility studies of current and novel photosensitizer drugs, absorbance and emission spectra measurements and fluorescence imaging
  • Optical phantom studies to mimic real tissues and organs to study organ-specific light propagation properties
  • Tissue heating studies such as laser interstitial thermal therapy (LITT)

Cell culture laboratory

  • Sterile area for cell culturing different cell types (e.g., cancer cells)
  • Study cell-drug interaction with current and new photosensitizers or other light activated drug delivery systems
  • Examine laser effect on real cell samples to determine optimal light dose for intended photodynamic effect

Clinical laser validation

  • Clinical laser systems (e.g. ML7710/ML6710i) available for treatment protocol, usability and validation testing
  • Light delivery system design and validation with phantoms, including combination to endoscopy and imaging
  • Clinical validation with local clinicians who often visit the factory to comment system design and usability

Modulight Biolab capabilities

General biolab capabilities

Fluorescence feasibility studies

  • Study photosensitizer optical properties
  • Analyze absorption and emission spectra
  • Fluorescence imaging of cell internalization

Laser light-tissue interaction

  • Laser – direct beam, fiber delivery or illumination kits
  • Laser thermal effects to tissue models
  • Tissue temperature monitoring (camera/thermocouplers/fiber probes)

Clinical laser testing

  • Ophthalmic laser device
    ML6710i
  • Clinical laser platform
    ML7710
  • Anatomical models (e.g., bladder)
  • Endoscopic light delivery and imaging/diagnosis

Cell culture capabilities

Optical phantom studies

  • Demonstrate light distribution in tissue with phantoms
  • Liquid phantoms: ink, Intralipid
  • Solid phantoms: agar, thermosensitive dye, ink, blood, Intralipid

Mechanistic studies of cancer cell responses to therapies

  • Determine PS uptake in cancer cells
  • Measure ROS production of photosensitizers
  • Determine mitochondrial membrane potential to photosensitizers

Cancer cell viability studies

  • Automated light activation and fluorescence monitoring with
    ML8500 (6/24/96 well plates)
  • Normoxia vs hypoxia testing
  • In-vitro cell viability testing of the cancer drugs (apoptosis/necrosis assays, MTT, Alamar Blue)
  • Microscopy of cells and/or cell viability assays

Advanced cancer cell studies

Liquid biopsy / immunological analysis

  • Analyze tumor cell profiles
  • Detect secreted proteins, e.g. cytokines (ELISA)
  • Detect presence of antibodies (e.g. COVID immunity)

Advanced in-vitro drug R&D

  • Determine cancer cell target inhibition efficacy by drugs
  • Biomarker detection
  • ELISA, ELFA, Alpha technology
  • Study protein/molecular binding
  • Drug solubility screening
  • Particle size & charge determination
  • Transcriptional activity of target protein

Lasers and illumination tools at Modulight biolab

Clinical laser platform ML7710

  • Ready for clinical and pre-clinical use
  • Can be tailored, support lasers in UV … 2+ µm
  • Spectral measurement from all channels individually, enabling
    • Photosensitizer presence confirmation
    • Photobleaching monitoring
    • Blood leakage detection
  • Remote support and diagnostics via Modulight Cloud

ML6600 IoT subsystem platform

  • Supporting different laser technologies: diode, SSL, fiber laser, VCSEL, VECSEL… in the range of UV … 2+ µm
  • Mechanics and connectivity can be tailored to enable integration into customer-specific setup
  • Preventive maintenance supported by Modulight Cloud

ML6710i ophthalmic laser

  • iPad controlled laser, integrated into ophthalmic treatment station and slit lamp
  • FDA approved for AMD treatments – 689 nm laser
  • Possible to customize for other indications / wavelengths

In vitro illumination chambers

  • Compatible with ML6600 and ML7710 lasers
  • Uniform light intensity over the whole area in the chamber
  • Can accommodate microplates and petri dishes

In vivo illumination setup

  • Adjustable height for in vivo tissue illumination studies
  • Laser light spot diameter can be modified
  • Compatible with ML6600 and ML7710 lasers

ML8500 – automatic biomedical illumination kit

  • Automatic sequential illumination of microplates using up to six wavelengths
  • Built-in temperature and atmospheric control
  • Supports making complex illumination programs in Modulight Cloud

Case examples

CASE: ML8500 for hypoxia

Hypoxia is a state in which oxygen is not available in sufficient amounts in the tissue. In vitro study conditions should ideally resemble physiological conditions as closely as possible (e.g. tumor centers are often hypoxic).

ML8500 has an incubation chamber optimized for hypoxia/normoxia experiments. Hypoxia dynamics, optimal gas flow rate, and different oxygen concentrations were optimized to be used for drug studies with ML8500.

CASE: ML8500 protocols for customer preclinical drug validation

Solution: Modulight carried out validation of customer’s protocol in Modulight’s BIOLAB using ML8500 tailored to customer’s final target specifications.

Execution: Customer sent Modulight cells and cell viability dye they are using in their validation protocol.

Result: Modulight carried out protocol in the biolab and prepared a comprehensive testing report for the customer.

CASE: Fluorescence feasibility study

Customer was interested in learning whether their photosensitive drug could be used also for photodynamic diagnosis, so Modulight agreed to perform characterization for the drug.

Work consisted of evaluating the optical properties of the drug by measuring its absorption and emission spectra.

Modulight lasers at different wavelengths were tested together with custom optical imaging filters to compare their feasibility for fluorescence excitation.

The effect of cell internalization was also studied to determine whether that has an impact on fluorescence signal intensity.

Based on these initial results, overall clinical program with this drug will be amended.

CASE: Fluorescence cystoscopy study

Anatomical bladder model, optimized for fluorescence cystoscopy studies.

ML7710 with 405nm laser perfectly suited for cystoscopy together with commercial PDD system, such as from Olympus or Karl Storz.

Tailored imaging optics for customer’s fluorescence drug to be used in bladder visualization and treatment.

ML7710 laser can be used also for PDT/PIT and treatment monitoring with any other drugs, and also to localize tumor using fluorescence diagnostics with either imaging dyes (like ICG, Hexvix) or the inherent fluorescence of the drug compound.

Related application notes

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