Automatic Biomedical Illumination System
ML8500 is the world's most versatile illumination device for in-vitro studies. ML8500 can support up to 7 different wavelengths simultaneously, flexible control of illumination parameters, and built-in incubation chamber for cells studies in normoxic and hypoxic conditions.
Automatic system for illuminating well plates
ML8500 is a great tool for sequential illumination of multi-well sample plates and optional fluorescence imaging/measurement. Prepare your samples, load into ML8500, and fully automatic system takes care of the rest. Operating the biomedical illumination system is very easy with an intuitive touch screen interface and Modulight’s cloud.
The system supports multiple illumination stations that illuminate different sized areas in the sample. For example, one station can be for Petri dish that is up to 95 mm in diameter and another for 96-well plates that illuminates each well separately with specific duration and intensity. Different illumination stations can be delivered for various well plates (24, 96, 384, 1536 wells) and a total of 4 illumination stations can be fitted in one system.
Device can be further equipped with environmental control for optimized cell-based assays. Hypoxia feature also enables conducting cell experiments in physiologically relevant conditions, since tumors are often hypoxic which affects the efficacy of oxygen-dependent therapies like photodynamic therapy.
Broad wavelength range support
Wavelengths in ML8500 selected to support most common PDT agents and fluorophores:
- 488 nm [fluorescein, Rose Bengal, methylene blue]
- 520 nm [TLD-1433, Rutherrin]
- 630 nm [Photofrin (porfimer sodium)]
- 635 nm [Gleolan (5-aminolevulinic acid)]
- 652 nm [Cy5, Foscan (thermoporfin), Radachlorin]
- 672 nm [Pc4 (silicon phthalocyanine 4)]
- 689 nm [IRDye700, Visudyne (verteporfin), Illuminox]
- 753 nm [Tookad (padeliporfin), AlexaFluor 750, Cy7]
- 800 nm [IRDye800, ICG (indocyanine green)]
One example configuration
Modulight Cloud Analytics and Services
Modulight Cloud connection enables real-time connectivity, treatment monitoring, remote support and diagnostics. Internet connectivity enables fluent transfer and viewing of the data and remote diagnostics via cloud service platform. The illumination protocols can be downloaded from the computer to Cloud, and fluorescence measurements are stored there in real time for further analysis. In addition, Cloud connectivity allows remote troubleshooting and updating of the instrument software. Diagnostics data can be viewed from cloud.modulight.com
- Device installation
- On-site training of personnel
- Periodic preventive maintenance
- Software updates and hardware upgrades
- On-site or remote technical support
- Support with application testing
- Treatment data analytics
- Lifecycle management / recycling
ML8500 case examples
ML8500 for hypoxia
Background: Hypoxia is a state in which oxygen is not available in sufficient amounts at the tissue level. In vitro study conditions should ideally resemble physiological conditions as closely as possible (e.g. tumor centers are often hypoxic)
Modulight solution with ML8500: ML8500 an incubation chamber optimized for hypoxic experiments. Hypoxia dynamics, optimal gas flow rate, and different oxygen concentrations were optimized to be used for drug studies with ML8500
Background: A novel photo-activated drug, HS Here, a novel targeted drug was developed for breast cancer by combining clinically approved verteporfin with a small molecule inhibitor of heat shock protein 90.
Modulight’s solution with ML8500: ML8500 was used to study optimal parameters for drug’s activation in a high-throughput manner. Highly aggressive MDA-MB-231 breast cancer cells were seeded on 96-well plates, incubated with different concentrations (0-3 µM) of either verteporfin or HS201, and illuminated with 689 nm wavelength laser at various doses (0-30 J/cm2) in different wells. Also different drug-light intervals (0, 3, and 6 hours) were studied with escalating light doses (0-120 J/cm2).
Background: Hypoxia presents a major challenge in the treatment of cancer, as low oxygen conditions make malignant tissues simultaneously more aggressive and less susceptible to standard treatments. To address this issue, a novel hypoxia-active photosensitive drug was developed for treatment of hypoxic tumors.
Modulight’s solution with ML8500: ML8500 provided optimal conditions for characterization of the drug using 525 nm and 630 nm wavelengths. In the first experiment, irradiance was constant at 300 mW/cm2 and fluence was varied between 10-300 J/cm2, while in the second experiment, fluence was at 100 J/cm2 and irradiance varied 25-300 mW/cm2 mW/cm2mW/cm2.
Background: The study of photoactivated drugs requires a controlled and systematic sample illumination process.
Modulight’s solution with ML8500: Novel photosensitive drug was characterized for a pharmaceutical company using ML8500 automated illumination system connected to ML7710 medical laser. The goal was to investigate the effect of irradiance and light dose on a cancer cell line while keeping the photosensitive drug dosing constant.
Motivation: Passive drug release from liposomes is erratic and inefficient, so light-triggered release offers attractive possibilities for efficient drug release at desired place and time.
Modulight’s solution with ML8500: Light-sensitive molecule indocyanine green was incorporated into liposome, releasing contents when illuminated with a laser light. Light-triggered release was studied by irradiating liposomes with ML8500 and was precisely controlled by strength and duration of the illumination.
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The laser light emitted from this laser device may be visible or invisible, depending on the laser selected. The laser light is harmful to the human eye. Avoid eye and skin exposure to the beam, both direct and reflected. Products are subject to the risks normally associated with sensitive electronic devices including static discharge, transients, and overload. Please ensure ESD protection prior to handling the products. The devices have built-in software and hardware based safety features required for medical purposes.