Fluorescence endoscopy
Fluorescence endoscopy is the latest advancement in endoscopy technology, which uses specialized dyes to highlight specific areas of interest and provide better visibility and image quality during procedures. This technology has revolutionized the way we view the inside of the body, enabling doctors to obtain more detailed images of the gastrointestinal tract, lungs, bladder, and reproductive organs.
Endoscopy
Endoscopy is a powerful tool in diagnosing and treating a wide range of medical conditions. With its minimally invasive approach and high accuracy, it has become a preferred option for many patients. Recent advances in imaging technology have made endoscopy even more effective. High-definition and 4K imaging, along with fluorescence imaging, have revolutionized the way we view the inside of the body.
One of the latest advancements in endoscopy technology is the development of miniaturized, single-use endoscopes. These devices offer a number of benefits over traditional endoscopes. Because they are disposable, there is no need for cleaning and sterilization after each use, which saves time and reduces the risk of infection. In addition, miniaturized endoscopes are less invasive and more comfortable for patients, making the procedure easier to tolerate. They are also more cost-effective, as there is no need for expensive reprocessing equipment. As endoscopy technology continues to evolve, there are increasing demands for improved illumination to provide better visibility and image quality during procedures. This is particularly important in fluorescence imaging, where specialized dyes are used to highlight specific areas of interest.
Doctors can obtain more detailed images of the gastrointestinal tract, lungs, bladder, and reproductive organs, leading to more accurate diagnoses and better treatment options for patients. With the ability to identify and remove polyps, foreign bodies, and tumors, endoscopy has become an indispensable tool for many medical professionals.
Fluorescence imaging
Fluorescence imaging relies on fluorescent dyes that are selectively taken up by certain tissues or structures, making them visible under certain wavelengths of light. To ensure optimal results, endoscopic illumination systems must precisely deliver light at the excitation wavelength corresponding to the dye used. Modulight’s laser technology offers a tailored solution, providing the specific wavelength required for precise fluorescence imaging.
In addition to specialized illumination systems, there are new developments in endoscopic technology designed to enhance fluorescence imaging. For example, endoscopes include built-in light filters that help to reduce background noise and improve image contrast, resulting in clearer images. As endoscopy technology advances, we can expect to see even more sophisticated illumination systems and imaging techniques that will further improve the accuracy and effectiveness of endoscopic procedures.
Modulight’s wavelength selection
Modulight offers multiple laser lines for exciting different fluorescent labels, each with its own absorption and emission characteristics, increasing the versatility and flexibility of the system. Most common wavelengths based on the antibody labels used in the biosciences field include: 405 nm, 445 nm, 488 nm, 532 nm, 561 nm, 633 nm, 640 nm, 660 nm, and 808 nm. Modulight covers the wavelength range from UV to 2000+ nm wavelengths, with many options to choose from for the new upcoming dyes in the biomedical field. Some example wavelength configurations for different fluorophores are shown in the table below.
Thanks to Modulight’s inhouse capabilities to manufacture lasers between UV and 2000+ nm, we can match these and many more fluorophore types, even uncommon and new ones.
| Fluorophore | Absorption (nm) | Emission (nm) |
| Alexa Fluor 405 | 401 | 442 |
| Pacific Blue | 404 | 455 |
| AmCyan | 457 | 489 |
| PerCP | 477 | 678 |
| EGFP | 489 | 510 |
| FITC | 495 | 519 |
| Alexa Fluor 488 | 499 | 520 |
| 7-AAD | 543 | 647 |
| Cy3 | 548 | 561 |
| PE-Cy5 | 565 | 665 |
| PE | 566 | 576 |
| PE-Cy7 | 566 | 778 |
| Alexa Fluor 568 | 577 | 603 |
| Alexa Fluor 594 | 590 | 618 |
| APC | 650 | 660 |
| Alexa Fluor 647 | 653 | 668 |
| Alexa Fluor 700 | 696 | 719 |
| APC-Cy7 | 756 | 779 |
| Alexa Fluor 790 | 782 | 805 |
| Fluorophore | Absorption (nm) | Emission (nm) |
| Indo-1 hi | 223 | 403 |
| Riboflavin | 266 | 531 |
| Cascade Yellow | 277 | 548 |
| Emerald 300 | 289 | 530 |
| QDot 525 | 300 | 525 |
| QDot 545 | 300 | 543 |
| QDot 565 | 300 | 564 |
| QDot 585 | 300 | 588 |
| QDot 605 | 300 | 603 |
| QDot 625 | 300 | 621 |
| QDot 655 | 300 | 654 |
| QDot 705 | 300 | 702 |
| QDot 800 | 300 | 792 |
| Fura-2 hi | 336 | 505 |
| FVS440UV | 336 | 435 |
| Alexa Fluor 350 | 342 | 441 |
| Indo-1 lo | 345–346 | 479 |
| AMCA | 346 | 442 |
| BUV395 | 347 | 393 |
| BUV496 | 350 | 491 |
| BUV563 | 350 | 560 |
| BUV615 | 350 | 661 |
| BUV661 | 350 | 653 |
| BUV737 | 350 | 732 |
| PI | 351 | 617 |
| BUV805 | 352 | 803 |
| Hoechst33258 | 352 | 455 |
| DAPI | 357–359 | 461 |
| Methyl Coumarin | 360 | 448 |
| Marina Blue | 364 | 460 |
| Fura-2 lo | 367 | 515 |
| DyeCycle Violet | 369 | 437 |
| FVS575V | 396 | 572 |
| Cascade Blue | 398 | 420 |
| Calcein Violet AM | 400 | 452 |
| Pacific Orange | 400 | 551 |
| Alexa Fluor 405 | 401 | 442 |
| BV510 | 402–407 | 510 |
| FVS450 | 404 | 448 |
| Pacific Blue | 404 | 455 |
| V450 | 405 | 450 |
| BV711 | 405–408 | 710 |
| BV570 | 407 | 573 |
| BV605 | 407 | 601 |
| BV650 | 407 | 646 |
| BV786 | 407 | 786 |
| BV750 | 407–408 | 745 |
| BV421 | 408 | 421 |
| V500 | 415 | 499 |
| FVS510 | 418 | 509 |
| Lucifer Yellow | 428 | 544 |
| Alexa Fluor 430 | 431 | 540 |
| Fura Red Hi | 434–435 | 666 |
| ECFP | 435 | 477 |
| BV480 | 436 | 476 |
| SYTOX Blue | 445 | 470 |
| AmCyan | 457 | 489 |
| DiA | 457 | 586 |
| Fura Red Lo | 471 | 672 |
| BB630P2 | 476 | 613 |
| BB660P2 | 476 | 658 |
| BB700 | 476 | 695 |
| BB755P | 476 | 758 |
| PerCP | 477 | 678 |
| PerCP-Cy5.5 | 480–482 | 676 |
| BB790P | 485 | 784 |
| DiO | 489 | 506 |
| EGFP | 489 | 510 |
| CF488A | 489–490 | 516 |
| BB515 | 490 | 513 |
| FVS520 | 491 | 517 |
| YO-PRO-1 | 491 | 506 |
| YOYO-1 | 491 | 508 |
| Calcein pH 9 | 493 | 514 |
| Fluo-4 | 494 | 516 |
| ZsGreen | 494 | 516 |
| FITC | 495 | 519 |
| Fluoro-Emerald | 495 | 523 |
| RB545 | 496 | 544 |
| Rho110 | 497 | 520 |
| Oregon Green | 498 | 526 |
| RB780 | 498 | 781 |
| SYBR Green | 498 | 522 |
| Alexa Fluor 488 | 499 | 520 |
| BODIPY | 502 | 511 |
| CyQUANT DNA | 502 | 523 |
| PicoGreen | 502 | 522 |
| Alexa Fluor 500 | 503 | 525 |
| SYTOX Green | 503 | 524 |
| DyeCycle Green | 506 | 534 |
| Fluo-3 | 506 | 527 |
| Na-Green | 506 | 531 |
| Magnesium Green | 507 | 531 |
| Rho123 | 507 | 529 |
| Via-Probe Green | 511 | 534 |
| TOTO-1 | 514 | 531 |
| EYFP | 515 | 528 |
| TO-PRO-1 | 515 | 531 |
| DyeCycle Orange | 519 | 563 |
| 6-JOE | 520 | 548 |
| FVS620 | 522 | 617 |
| EB | 523 | 604 |
| Eosin | 525 | 546 |
| Alexa Fluor 532 | 528 | 552 |
| BODIPY R6G | 528 | 547 |
| mBanana | 540 | 553 |
| 7-AAD | 543 | 647 |
| SYTOX Orange | 547 | 569 |
| Cy3 | 548 | 561 |
| mOrange | 548 | 562 |
| SNARF-1 (pH6) | 549 | 586 |
| DiI | 551 | 569 |
| CoralHue mk02 | 552 | 565 |
| Kusabira-Orange | 552 | 565 |
| DsRED | 553 | 585 |
| FVS570 | 553 | 578 |
| TMRho | 553 | 581 |
| CF555 | 554 | 568 |
| dTomato | 554 | 581 |
| Cy3B | 559 | 571 |
| Nile Red | 559 | 637 |
| RY586 | 564 | 588 |
| BYG584P | 563–564 | 582 |
| PE-Cy5 | 565 | 665 |
| PE-Cy5.5 | 565 | 693 |
| PE | 566 | 576 |
| PE-CF594 | 566 | 612 |
| PE-Cy7 | 566 | 778 |
| PE-Texas Red | 566 | 616 |
| mTangerine | 568 | 585 |
| Rhodamine Red | 573 | 591 |
| mStrawberry | 574 | 596 |
| SNARF-1 (pH9) | 576 | 640 |
| Cy3.5 | 576–579 | 589 |
| Alexa Fluor 568 | 577 | 603 |
| MitoTracker Red | 578 | 598 |
| mCherry | 587 | 610 |
| mPlum | 587 | 650 |
| Alexa Fluor 594 | 590 | 618 |
| JC-1 | 593 | 595 |
| Texas Red | 595 | 613 |
| mRaspberry | 597 | 624 |
| YOYO-3 | 612 | 631 |
| YO-PRO-3 | 613 | 629 |
| Phycocyanin | 617 | 646 |
| Alexa Fluor 633 | 631 | 647 |
| Via-Probe Red | 631 | 651 |
| SYTOX Red | 640 | 658 |
| TO-PRO-3 | 642 | 657 |
| TOTO-3 | 642 | 661 |
| Cy5 | 647 | 662 |
| DRAQ5 | 647 | 683 |
| DiD | 648 | 670 |
| APC | 650 | 660 |
| CF647 | 652 | 668 |
| Alexa Fluor 647 | 653 | 668 |
| APC-R700 | 653 | 706 |
| FVS660 | 656 | 670 |
| FVS700 | 656 | 697 |
| Alexa Fluor 660 | 663 | 691 |
| CF660 | 666 | 685 |
| Alexa Fluor 680 | 679 | 702 |
| CF680 | 681 | 689 |
| R718 | 694–697 | 715 |
| Alexa Fluor 700 | 696 | 719 |
| DiR | 750 | 782 |
| Alexa Fluor 750 | 752 | 779 |
| Cy7 | 753 | 775 |
| CF750 | 755 | 779 |
| APC-Cy7 | 756 | 779 |
| FVS780 | 757 | 778 |
| APC-H7 | 759 | 782 |
| CF770 | 770 | 797 |
| Alexa Fluor 790 | 782 | 805 |
Modulight solutions for endoscopic imaging
Fluorescence imaging is a powerful technique that utilises fluorescent molecules to visualise specific biological structures or molecules within a sample. Modulight’s ML6600 and ML7710 are advanced solutions for fluorescence imaging in endoscopy.
Overall, Modulight’s fluorescence imaging solutions offer researchers and clinicians reliable and user-friendly platforms for their fluorescence imaging needs, enabling them to perform a wide range of applications in various fields, such as medical research, drug development, and diagnostics. At Modulight, we are proud to offer the most advanced illumination technology for fluorescence imaging in endoscopy procedures.
Modulight’s operations are ISO13485 certified, ensuring that our products meet the highest quality and safety standards. Our customers include world-leading cancer centers and pharmaceutical companies, who trust our technology for their most demanding endoscopy applications. Our commitment to innovation and customer satisfaction makes Modulight the trusted choice for high-quality fluorescence imaging in endoscopy procedures.
Cloud-powered laser solution platform ML6600 - easy to integrate
The ML6600 offers a compact package with all the necessary wavelengths, providing precise and noise-free illumination. It integrates essential elements into a single device, making it ideal for 4K image processing and miniaturized single-use endoscopy applications. ML6600 can be customized and it’s easy to integrate as a part of a larger system.
Modulight ML6600 based illumination platform is a complete light engine solution, incorporating in-house lasers, electronics, and optics. This enables us to provide also a fully customized solution for each customer, tailored to their specific requirements. We offer a range of wavelength options, including blue light and near-infrared light, to accommodate a variety of fluorescent dyes and agents. Our technology can be integrated into both laboratory sources and OEM products, making it a versatile and cost-effective solution for endoscopy applications.
“With Modulight ML6600 innovative technology, doctors can be confident that they are receiving the most detailed and accurate images possible, leading to better patient outcomes and improved healthcare overall.”
Clinical laser platform ML7710 - next-generation light source with feedback signal monitoring
The ML7710 is a next-generation clinical laser platform that delivers white light and customized fluorescence wavelengths in one compact package. It also offers optional spectral fluorescence detection, enabling the detection of novel dyes not supported by commercial instruments. Designed in collaboration with end users, the ML7710 ensures optimal clinical usability and provides a versatile solution for advanced endoscopic fluorescence imaging.
Modulight’s ML7710 can also be used for monitoring light-based treatments with the help of monitoring changes in the fluorescence emission of the drug. For example drugs used in photoimmunotherapy typically have intrinsic fluorescence properties when excited with specific wavelength of light. Inducing fluorescence and monitoring photobleaching of the drug can help to verify that the drug is localized in the tissue and adjusting the light delivery time to be optimal for each individual patient, in accordance with the approach of personalized medicine.
“The Modulight ML7710 is a game-changer for fluorescence endoscopy. Its advanced light source, offering both white light and customized laser wavelengths mathing the fluorescence absorption maximums, revolutionizes tumor visualization and diagnostics during endoscopic procedures. With its precise excitation and high fluorescence excitation efficiency, the ML7710 enables enhanced detection of malignant lesions and improved intraoperative visualization. It’s a powerful tool that empowers clinicians to achieve more accurate and comprehensive tumor removal, ultimately leading to better patient outcomes.”
Read more:
Application note: Fluorescence imaging & endoscopy for tumor visualization and diagnostics
Endoscopic imaging is typically performed using normal white light. However, certain tumors can be difficult to detect under white light since all structures are illuminated without specificity to malignant lesions. Fluorescence endoscopy is for this reason increasingly used in cancer diagnostics and surveillance to improve detection of cancerous and precancerous lesions. Special fluorescent dyes are normally administered to patient and preferentially accumulate into malignant lesions and this way increase contrast between malignant and heathy tissues when excited when specific wavelength of light.
ML6600: Cloud-powered laser solution platform
ML6600 is a compact, easy-to-integrate laser solution that can be flexibly tailored to various applications, such as Flow Cytometry, Semiconductor Metrology, Quantum Computing, Diagnostics, Genetics, and various medical applications.
It’s well suited for medical system integrators, as it’s designed in accordance with IEC 60601-1 norm. This will speed up the certification process. The lasers and the system are manufactured in ISO13485 certified fab.
ML7710: Clinical laser platform
Modulight’s ML7710 next-generation light source for endoscopy delivers both white light and separate fluorescence wavelengths as customized for each use case, providing compact design for all light sources in one package. ML7710 can also include optional spectral fluorescence detection within the same unit. This is especially useful functionality in the development of novel dyes that are not yet supported by the optics of the commercial instruments to detect fluorescence. The system has been designed in close collaboration with end users, ensuring optimal clinical usability.