Tag Archives: PACT

Phototruncation cell tracking with near-infrared photoimmunotherapy using heptamethine cyanine dye to visualise migratory dynamics of immune cells

Published in: eBioMedicine Authors: Hiroshi Fukushima, Aki Furusawa, Seiichiro Takao, Siddharth S. Matikonda, Makoto Kano, Shuhei Okuyama, Hiroshi Yamamoto, Peter L. Choyke, Martin J. Schnermann, Hisataka Kobayashi  Published in: eBioMedicine Authors: Hiroshi Fukushima, Aki Furusawa, Seiichiro Takao, Siddharth S. Matikonda, Makoto Kano, Shuhei Okuyama, Hiroshi Yamamoto, Peter L. Choyke, Martin J. Schnermann, Hisataka Kobayashi A new method called phototruncation-assisted cell tracking (PACT) can be used to noninvasively track migration of immune cells to understand anti-cancer immunity mechanisms. PACT is based on irreversible photo-induced truncation reaction, transforming Cy7 into Cy5 when exposed to NIR light (780 nm). PACT was used in this study to monitor spatiotemporal migration of immune cells between tumor and Continue reading →

Modulight Spotlights: LASER-SHARP RESEARCH – February 2024

 Modulight Spotlights: LASER-SHARP RESEARCH – February 2024 Photoactivated chemotherapy (PACT) is a novel anticancer therapy where cytotoxic drug is attached into a photocage, which is cleaved by light to release the drug at the tumor site. A new study led by Prof. Sylvestre Bonnet’s group at Leiden University, demonstrated a significantly improved agent for photoactivated chemotherapy. ML6600 laser was used to study the drug release from the photocage, comparing efficacy between red and green light. It was shown that the agent could be effectively released Continue reading →

Red-Light Activation of a Microtubule Polymerization Inhibitor via Amide Functionalization of the Ruthenium Photocage

Published in: Angew Chem Int Ed Authors: Ludovic Bretin, Yurii Husiev, Vadde Ramu, Liyan Zhang, Matthijs Hakkennes, Selda Abyar, Andrew C. Johns, Sylvia E. Le Dévédec, Tania Betancourt, Alexander Kornienko, Sylvestre Bonnet Leiden University A ruthenium-based compound was developed for PACT (photoactivated chemotherapy), which unlike most existing PACT compounds, can be also activated with clinically relevant longer (red) wavelength of light. ML6600 was used for in vivo experiments to superficially activate PACT compound in subcutaneous tumor comparing green and red wavelengths. The tumor volume reduction was Continue reading →

Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

Published in: Journal of the American Chemical Society Authors: Hiroshi Fukushima, Siddharth S. Matikonda, Syed Muhammad Usama, Aki Furusawa, Takuya Kato, Lenka Štacková, Petr Klán, Hisataka Kobayashi, Martin J. Schnermann  Published in: Journal of the American Chemical Society Authors: Hiroshi Fukushima, Siddharth S. Matikonda, Syed Muhammad Usama, Aki Furusawa, Takuya Kato, Lenka Štacková, Petr Klán, Hisataka Kobayashi, Martin J. Schnermann In this study, phototruncation-assisted cell tracking (PACT) was developed to enable spatiotemporal tracking of immune cell populations in vivo. It is based on cyanine photoconversion reaction that leads to 2-carbon truncation and blue shift in the emission wavelength when irradiated with NIR light. ML6600 (780 nm) was used to induce photoconversion both in vitro and Continue reading →