Microscopy
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye. There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Versatility and flexibility of microscopes is achieved by introducing multiple laser wavelengths to target existing and newly developed fluorophores.
Microscopy
Microscopy provides unparalleled insights into the micro-world, enabling high-resolution imaging for scientific research and medical diagnosis. One of the most significant breakthroughs in microscopy is the development of high-resolution and multi-modal imaging techniques. Techniques such as confocal microscopy, super-resolution microscopy, and multiphoton microscopy have significantly improved image clarity and detail.
Advancements in illumination techniques have played a crucial role in enhancing microscopy capabilities. Techniques such as fluorescence microscopy and light sheet microscopy enable researchers to visualize specific molecules or structures within biological samples with exceptional sensitivity and specificity. These techniques have revolutionized biomedical research by facilitating the study of dynamic processes within living organisms. Simultaneously, the demands on the laser light engines have increased. Modulight has gladly accepted the challenge and successfully responded to these growing needs.
Microscopy enables researchers and healthcare professionals to obtain detailed images of cells, tissues, and microorganisms, facilitating the diagnosis and treatment of various diseases. Whether examining tissue samples for cancerous cells or studying the structure of nanomaterials, microscopy continues to be an indispensable tool in advancing scientific knowledge and improving human health.
Fluorescence microscopy
❑ Confocal (laser scanning) microscopy is widely used in biomedical research
❑ Tissue or cell samples are labeled with fluorescent dyes that can be fluorescently identified or tracked even in live cells
❑ Images at different depths can be used to reconstruct extremely high-quality 3D images
❑ Main application fields are histopathology, immunofluorescence, and cellular biology
Why to choose Modulight for fluorescence microscopy:
❑ Single unit, multiple wavelengths
❑ Compactness
❑ Integration
❑ Miniaturization
❑ Tunable and scalable powers
❑ De-speckling
❑ Beam shaping optics
❑ Tailorable wavelengths to match all fluorophores
Modulight solution for microscopy
Modulight is specialized to provide whole illumination solution for microscopy, with all the needed wavelengths in a compact, easy-to-integrate single package, the ML6600
Modulight Solution for Microscopy: ML6600
Modulight is specialized to provide whole illumination solution for microscopy, providing focused, noise-free, and monochromatic illumination.
The ML6600 for microscopy introduces a high level of integration and miniaturization of the light engine elements in microscope.
Optional fiber coupled solution enables easy optical integration.
Beam shaping optics and de-speckling can be integrated.
Maintenance service (i.e., annual calibrations) is easily supported thanks to the Cloud connectivity of ML6600.
Predictive maintenance is supported both on-site and off-site.
Cloud-powered laser solution platform ML6600 - easy to integrate
The multi-wavelength ML6600 platform integrates all the core optical elements into one single box providing the customer a single optical arrangement solution with all the most important wavelengths in a compact package. Product platform was originally launched in 2013. ML6600 can host different laser technologies (diode, fiber, DPSS). Wavelength can be tailored from UV up to 2 µm and power levels from mWs to Ws to your application.
- Speed up your certification: designed in accordance to IEC 60601-1:
- Safety features to support risk control measures of your medical system.
- Manufactured in ISO13485 certified fab
- ML6600 is a platform product, which enables flexible tailoring to OEM customers’ needs.
- Preventive maintenance with predictive analytics and machine learning algorithms, powered by Modulight Cloud.
- Applications of ML6600 include Microscopy, Flow Cytometry, Semiconductor Metrology, Quantum computing, Diagnostics, Genetics, etc.
- Plug and play: included calibration data
- Standard interlock interface
- PC control interface
- Preventive maintenance
- External trigger
- RS422 or Ethernet
- Integrated laser driver
- Passive or active integrated cooling
- 1-8 output channels
- Compact size & tailored mechanics
- Tailorable for wide variety of applications
- Environment & laser status
ML6600 is a completely customizable and easy-to-integrate laser platform.
Examples of supported wavelengths
Thanks to Modulight’s inhouse capabilities to manufacture lasers between UV and 2000+ nm, we can match these and many more fluorophore types, even uncommon and new ones.
| Fluorophore | Absorption (nm) | Emission (nm) |
| Alexa Fluor 405 | 401 | 442 |
| Pacific Blue | 404 | 455 |
| AmCyan | 457 | 489 |
| PerCP | 477 | 678 |
| EGFP | 489 | 510 |
| FITC | 495 | 519 |
| Alexa Fluor 488 | 499 | 520 |
| 7-AAD | 543 | 647 |
| Cy3 | 548 | 561 |
| PE-Cy5 | 565 | 665 |
| PE | 566 | 576 |
| PE-Cy7 | 566 | 778 |
| Alexa Fluor 568 | 577 | 603 |
| Alexa Fluor 594 | 590 | 618 |
| APC | 650 | 660 |
| Alexa Fluor 647 | 653 | 668 |
| Alexa Fluor 700 | 696 | 719 |
| APC-Cy7 | 756 | 779 |
| Alexa Fluor 790 | 782 | 805 |
Thanks to Modulight’s inhouse capabilities to manufacture lasers between UV and 2000+ nm, we can match these and many more fluorophore types, even tailored, uncommon and new dyes.
| Fluorophore | Absorption (nm) | Emission (nm) |
| Indo-1 hi | 223 | 403 |
| Riboflavin | 266 | 531 |
| Cascade Yellow | 277 | 548 |
| Emerald 300 | 289 | 530 |
| QDot 525 | 300 | 525 |
| QDot 545 | 300 | 543 |
| QDot 565 | 300 | 564 |
| QDot 585 | 300 | 588 |
| QDot 605 | 300 | 603 |
| QDot 625 | 300 | 621 |
| QDot 655 | 300 | 654 |
| QDot 705 | 300 | 702 |
| QDot 800 | 300 | 792 |
| Fura-2 hi | 336 | 505 |
| FVS440UV | 336 | 435 |
| Alexa Fluor 350 | 342 | 441 |
| Indo-1 lo | 345–346 | 479 |
| AMCA | 346 | 442 |
| BUV395 | 347 | 393 |
| BUV496 | 350 | 491 |
| BUV563 | 350 | 560 |
| BUV615 | 350 | 661 |
| BUV661 | 350 | 653 |
| BUV737 | 350 | 732 |
| PI | 351 | 617 |
| BUV805 | 352 | 803 |
| Hoechst33258 | 352 | 455 |
| DAPI | 357–359 | 461 |
| Methyl Coumarin | 360 | 448 |
| Marina Blue | 364 | 460 |
| Fura-2 lo | 367 | 515 |
| DyeCycle Violet | 369 | 437 |
| FVS575V | 396 | 572 |
| Cascade Blue | 398 | 420 |
| Calcein Violet AM | 400 | 452 |
| Pacific Orange | 400 | 551 |
| Alexa Fluor 405 | 401 | 442 |
| BV510 | 402–407 | 510 |
| FVS450 | 404 | 448 |
| Pacific Blue | 404 | 455 |
| V450 | 405 | 450 |
| BV711 | 405–408 | 710 |
| BV570 | 407 | 573 |
| BV605 | 407 | 601 |
| BV650 | 407 | 646 |
| BV786 | 407 | 786 |
| BV750 | 407–408 | 745 |
| BV421 | 408 | 421 |
| V500 | 415 | 499 |
| FVS510 | 418 | 509 |
| Lucifer Yellow | 428 | 544 |
| Alexa Fluor 430 | 431 | 540 |
| Fura Red Hi | 434–435 | 666 |
| ECFP | 435 | 477 |
| BV480 | 436 | 476 |
| SYTOX Blue | 445 | 470 |
| AmCyan | 457 | 489 |
| DiA | 457 | 586 |
| Fura Red Lo | 471 | 672 |
| BB630P2 | 476 | 613 |
| BB660P2 | 476 | 658 |
| BB700 | 476 | 695 |
| BB755P | 476 | 758 |
| PerCP | 477 | 678 |
| PerCP-Cy5.5 | 480–482 | 676 |
| BB790P | 485 | 784 |
| DiO | 489 | 506 |
| EGFP | 489 | 510 |
| CF488A | 489–490 | 516 |
| BB515 | 490 | 513 |
| FVS520 | 491 | 517 |
| YO-PRO-1 | 491 | 506 |
| YOYO-1 | 491 | 508 |
| Calcein pH 9 | 493 | 514 |
| Fluo-4 | 494 | 516 |
| ZsGreen | 494 | 516 |
| FITC | 495 | 519 |
| Fluoro-Emerald | 495 | 523 |
| RB545 | 496 | 544 |
| Rho110 | 497 | 520 |
| Oregon Green | 498 | 526 |
| RB780 | 498 | 781 |
| SYBR Green | 498 | 522 |
| Alexa Fluor 488 | 499 | 520 |
| BODIPY | 502 | 511 |
| CyQUANT DNA | 502 | 523 |
| PicoGreen | 502 | 522 |
| Alexa Fluor 500 | 503 | 525 |
| SYTOX Green | 503 | 524 |
| DyeCycle Green | 506 | 534 |
| Fluo-3 | 506 | 527 |
| Na-Green | 506 | 531 |
| Magnesium Green | 507 | 531 |
| Rho123 | 507 | 529 |
| Via-Probe Green | 511 | 534 |
| TOTO-1 | 514 | 531 |
| EYFP | 515 | 528 |
| TO-PRO-1 | 515 | 531 |
| DyeCycle Orange | 519 | 563 |
| 6-JOE | 520 | 548 |
| FVS620 | 522 | 617 |
| EB | 523 | 604 |
| Eosin | 525 | 546 |
| Alexa Fluor 532 | 528 | 552 |
| BODIPY R6G | 528 | 547 |
| mBanana | 540 | 553 |
| 7-AAD | 543 | 647 |
| SYTOX Orange | 547 | 569 |
| Cy3 | 548 | 561 |
| mOrange | 548 | 562 |
| SNARF-1 (pH6) | 549 | 586 |
| DiI | 551 | 569 |
| CoralHue mk02 | 552 | 565 |
| Kusabira-Orange | 552 | 565 |
| DsRED | 553 | 585 |
| FVS570 | 553 | 578 |
| TMRho | 553 | 581 |
| CF555 | 554 | 568 |
| dTomato | 554 | 581 |
| Cy3B | 559 | 571 |
| Nile Red | 559 | 637 |
| RY586 | 564 | 588 |
| BYG584P | 563–564 | 582 |
| PE-Cy5 | 565 | 665 |
| PE-Cy5.5 | 565 | 693 |
| PE | 566 | 576 |
| PE-CF594 | 566 | 612 |
| PE-Cy7 | 566 | 778 |
| PE-Texas Red | 566 | 616 |
| mTangerine | 568 | 585 |
| Rhodamine Red | 573 | 591 |
| mStrawberry | 574 | 596 |
| SNARF-1 (pH9) | 576 | 640 |
| Cy3.5 | 576–579 | 589 |
| Alexa Fluor 568 | 577 | 603 |
| MitoTracker Red | 578 | 598 |
| mCherry | 587 | 610 |
| mPlum | 587 | 650 |
| Alexa Fluor 594 | 590 | 618 |
| JC-1 | 593 | 595 |
| Texas Red | 595 | 613 |
| mRaspberry | 597 | 624 |
| YOYO-3 | 612 | 631 |
| YO-PRO-3 | 613 | 629 |
| Phycocyanin | 617 | 646 |
| Alexa Fluor 633 | 631 | 647 |
| Via-Probe Red | 631 | 651 |
| SYTOX Red | 640 | 658 |
| TO-PRO-3 | 642 | 657 |
| TOTO-3 | 642 | 661 |
| Cy5 | 647 | 662 |
| DRAQ5 | 647 | 683 |
| DiD | 648 | 670 |
| APC | 650 | 660 |
| CF647 | 652 | 668 |
| Alexa Fluor 647 | 653 | 668 |
| APC-R700 | 653 | 706 |
| FVS660 | 656 | 670 |
| FVS700 | 656 | 697 |
| Alexa Fluor 660 | 663 | 691 |
| CF660 | 666 | 685 |
| Alexa Fluor 680 | 679 | 702 |
| CF680 | 681 | 689 |
| R718 | 694–697 | 715 |
| Alexa Fluor 700 | 696 | 719 |
| DiR | 750 | 782 |
| Alexa Fluor 750 | 752 | 779 |
| Cy7 | 753 | 775 |
| CF750 | 755 | 779 |
| APC-Cy7 | 756 | 779 |
| FVS780 | 757 | 778 |
| APC-H7 | 759 | 782 |
| CF770 | 770 | 797 |
| Alexa Fluor 790 | 782 | 805 |
Read more:
Application note: Customized ML6600 for multimodal imaging microscope for tissue engineering applications
Customer needed a high-resolution 3D imaging microscope to visualize the development and composition of tissue constructs made by tissue engineering. Modulight developed a 3-wavelength illumination module based on ML6600 technology. ML6600-based illumination module was used for selective plane illumination microscopy (SPIM) with 488, 561, and 638nm lasers for efficient fluorophore excitation. Laser was specifically designed to be stable even at very low powers (<40 mW) to enable longer periods of continuous imaging without fast photobleaching and phototoxicity to the sample.
ML6600: Cloud-powered laser solution platform
ML6600 is a compact, easy-to-integrate laser solution that can be flexibly tailored to various applications, such as Flow Cytometry, Semiconductor Metrology, Quantum Computing, Diagnostics, Genetics, and various medical applications.
It’s well suited for medical system integrators, as it’s designed in accordance with IEC 60601-1 norm. This will speed up the certification process. The lasers and the system are manufactured in ISO13485 certified fab.
Application note: Fluorescence imaging & endoscopy for tumor visualization and diagnostics
Endoscopic imaging is typically performed using normal white light. However, certain tumors can be difficult to detect under white light since all structures are illuminated without specificity to malignant lesions. Fluorescence endoscopy is for this reason increasingly used in cancer diagnostics and surveillance to improve detection of cancerous and precancerous lesions. Special fluorescent dyes are normally administered to patient and preferentially accumulate into malignant lesions and this way increase contrast between malignant and heathy tissues when excited when specific wavelength of light.