Laser Illumination: In Vitro and In Vivo
Modulight offers a complete portfolio of laser systems for in vitro and in vivo illumination. Our lasers are trusted worldwide in many cell research laboratories, drug discovery studies, and requiring photodynamic therapy (PDT) and photoimmunotherapy (PIT) research. Our products enable precise light delivery from UV wavelengths to more than 3000 nm, matching commercial photosensitizers and fluorescent dyes.
Photosensitizers are widely used in photodynamic therapy (PDT), photoimmunotherapy (PIT), and targeted drug delivery, where light precisely activates or releases therapeutic agents at the disease site.
In parallel, fluorescent dyes are essential markers in both in-vitro (e.g. microscopy, flow cytometry, cell assays) and in-vivo (e.g. imaging, diagnostics, pre-clinical models) applications. These enable breakthroughs in cancer treatment, drug discovery, and biomedical imaging.
Modulight’s in-vitro and in-vivo laser platforms provide wavelengths from UV to over 3000 nm, covering all known photosensitizers and fluorescent dyes. This versatility supports research across PDT, PIT, drug development, medical imaging, and advanced microscopy.
From In Vitro to Clinical applications with Modulight lasers
In vitro research
In-vitro illumination is used for studying cells on well plates or petri dishes with precise light delivery. Typical experiments include dose-escalation studies, where both drug and light dose are varied and combined with cell viability assays. Modulight lasers also support drug discovery by enabling light-activated release from carriers such as liposomes. These methods are widely applied in cancer drug development and evaluation of new photosensitizers and fluorescent dyes.
In vivo research
In-vivo illumination brings light-based research into animal models and pre-clinical studies. Applications range from tumor growth inhibition with PDT and PIT to testing light-activated drug delivery concepts. Fluorescent dyes are commonly used for real-time imaging and tracking biological processes. Modulight’s broad wavelength coverage ensures compatibility with all major markers and enables accurate, reproducible illumination for translational research.
Clinical
In clinical settings, Modulight lasers are applied in treatments such as PDT for cancers including glioblastoma, choroidal melanoma, and lung cancer. Light can be precisely directed to activate photosensitizers at the disease site, minimizing damage to healthy tissue. Beyond PDT, clinical research explores PIT, image-guided therapies, and integration with advanced diagnostic tools. Modulight platforms are designed to meet medical device standards, ensuring safe and effective use in patient care.
Modulight product offering for in vitro and in vivo applications
ML8500 - automated sequential laser illumination
The ML8500 is the most versatile in-vitro illumination system, supporting up to 7 simultaneous wavelengths. It features touchscreen control, precise dose management, and an integrated incubator for normoxia and hypoxia experiments. The system enables sequential illumination of well plates (24–1536 wells) and petri dishes, with optional cloud connectivity for remote operation and data logging.
ML6600 - cloud-connected laser platform
The ML6600 is a customizable, cloud-connected laser platform covering a wide spectral range from UV to over 3000 nm. It integrates multiple laser technologies, including diode, VECSEL, DPSSL, and fiber lasers. Application-specific versions are available — for example, flow cytometry models with a built-in CCD camera and AI-based active beam alignment, or microscopy models offering key wavelengths (355, 405, 488, 532, 561, 638 nm) with high uniformity and coherence.
Illumination kit - flexible in vivo and in vitro illumination tools
The Modulight Illumination Kit provides uniform and reproducible light delivery for well plates, petri dishes, and other sample formats. It is fully compatible with the ML6000 and ML7000 series lasers, enabling multi-wavelength experiments for fluorescence, PDT, and other biomedical applications.
ML6710i - Ophthalmic PDT laser
The ML6710i is an FDA-approved ophthalmic laser system designed for photodynamic therapy (PDT) with Visudyne® at 689 nm. It features intuitive iPad control, secure cloud-based treatment logging, and a beam-shaper accessory for uniform, circular beam profiles with all major slit lamps. The system can also be customized to support a wide range of wavelengths from UV to over 3000 nm.
ML7710 - multi-indication oncology laser platform
The ML7710 is a configurable, multichannel clinical laser platform covering UV to 3000 nm with up to 8 independent channels. It supports all major photosensitizers (e.g., 5-ALA, Verteporfin, Metvix, Foscan, IRDye 700) and is widely used in PDT, PIT, and advanced biomedical imaging. Features include touchscreen operation, cloud connectivity, self-calibration, safety interlocks, treatment monitoring, and optional fluorescence and photobleaching tracking — paving the way for future AI/ML-assisted therapy optimization.
Application Notes and Articles by Our Customers
Deeksha Sankepalle and the group at Tufts University, USA developed an advanced multi-wavelength photoacoustic imaging system. It integrates a pulsed, tunable laser with an ultrasound acquisition platform to provide functional 3D imaging. The system enables real-time, non-invasive, high-resolution imaging of tumor vasculature by tracking oxygen saturation in the tumor.
Modulight Spotlights: LASER-SHARP RESEARCH – November 2025 We are delighted to highlight new exciting study by Rahman et al. from Dr. Huang-Chiao Huang’s lab at University of Maryland. Multidrug resistance remains a significant barrier to successful chemotherapy outcomes for cancer patients, often caused by active pumping of chemotherapeutics outside of cancer cells. Hence, the team [...]
Published in: Cancer Immunology, Immunotherapy Authors: Motofumi Suzuki, Aki Furusawa, Hiroshi Yamamoto, Makoto Kano, Miyu Kano, Seiichiro Takao, Shuhei Okuyama, Peter L. Choyke, Hisataka Kobayashi
The spotlight of the month goes to Andrew Langley et al. at Tufts University, USA, for their impressive work in leveraging photoacoustic imaging for real-time monitoring of phototherapy. Photoacoustic imaging is 3D, non-ionizing and non-invasive imaging method, based on optical absorption that creates pressure waves detected by ultrasound transducer.
Published in: Langmuir Authors: Sumiao Pang, Rebecca J. Johnson, Nikhil Pandey, Anshika Kapur, Pavlos Anastasiadis, Pranjali Kanvinde, Emylee McFarland, Jeffrey A. Winkles, Graeme F. Woodworth, Anthony J. Kim, Huang Chiao Huang
Published in: npj Acoustics Authors: Andrew Langley, Allison Sweeney, Ronak T. Shethia, Brooke Bednarke, Faizah Wulandana, Marvin Xavierselvan, Srivalleesha Mallidi
Watch video about Modulight's In Vitro / In Vivo Solutions
Illumination solution can be customized to your needs:
- Wavelength can be selected between UV – 3000+ nm
- Irradiance and dose can be adjusted
- Different sample stages supporting the use of microplates, petri dishes, and possibility for customized
- Handpiece enables in vivo subcutaneous illumination, option for spot size adjustment
- We provide many different medical fiber options
- Controlled and repeatable illumination, support for dose calculation
- Possibility to use 1-8 different wavelengths
- Pulsing possible in ms – µs range
- On-site training
- Regular calibrations and device support
In-vitro and in-vivo customer referrals
Poul Martin Bendix, PhD, Associate Professor at Niels Bohr Institute
Sherri McFarland, PhD, Professor at University of Texas at Arlington (Formerly Professor at The University of North Carolina at Greensboro)
Discover Modulight Medical Lasers
Get the official brochure with product features, specifications, and clinical applications in oncology, ophthalmology, and research.
